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Rooting the rootless plant directly into the external adaption media in tissue culture propagation

Friday, 08 September 2017 03:19 Written by 
  • Location(s): Turkey
  • Type(s): Solution
  • Theme(s): Agriculture , Horticulture
  • SDG(s): 8. Decent Work and Economic
  • Locations of Agro Solutions: Turkey
  • Types of Agro Solutions: Solution
  • Themes in Agro Solutions: Agriculture , Horticulture
  • SDGs in Agro Solutions: 8. Decent Work and Economic
  • Locations in Europe and the CIS: Turkey
  • Types in Europe and the CIS: Solution
  • Themes in Europe and the CIS: Agriculture , Horticulture
  • SDGs in Europe and the CIS: 8. Decent Work and Economic

The traditional tissue culture technique, a widely used method for the mass production of fruit tree rootstocks, consists of 5 stages: development of shoots from explants, tillering, shoot elongation, rooting to a medium and the external adaption media. It’s costly as involves laboratory infrastructure investment costs, labor, chemical consumables and energy costs. in order to reduce the cost of production, in 2016 the Aegean Agricultural Research Institute developed a solution on rooting the rootless plant directly into the external adaption media in tissue culture propagation.

The solution is a tissue culture technique, where the rootless plantlets in the elongation medium is taken directly to the external adaption media (in greenhouse-containing 85-90% moist air) instead of the rooting medium. The solution is to run in the external adaption media where the cost of rooting is lower than that of rooting application in laboratories. By eliminated a stage, where the rootless plantlets in the elongation medium is taken to the rooting medium, the time for production within the laboratory is shortened while saving labor, chemical consumables and energy; the amount of production per unit area is increased.

Benefits and advantages of the solution:

  • reducing investment costs for laboratory production by 20%.
  • reducing production cost by 30%.
  • shortening of the production period by 30%.
  • increasing of the plant production per unit of area by 30%.
  • lab area requirement less by 30%.
  • labor requirement less by 20%
  • chemical consumables requirement less by 30%.
  • Energy-saving by 15%.

Achievements: 3 companies that produce rootstocks by tissue culture, including Bademli biotechnology laboratory, started implementing the solution and reduced the cost of nursery plant production by an average of 15%.

Partners: The Ministry of Food, Agriculture and Livestock, the private farmers and companies.

Contact information:
Turkey, Aegean Agricultural Research Institute, Menemen, İzmir, Turkey
Contact person: Andac Cavdar (Msc) Erol Kucuk (PhD),
Telephone: +905055730650, +905308849362
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;  This email address is being protected from spambots. You need JavaScript enabled to view it.

Read 2972 times Last modified on Saturday, 23 December 2017 09:21
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